Citrate agar slants contain sodium citrate, bromthymol blue (pH indicator), sodium, and water. If an organism is able to utilize citrate, an enzyme called citrase will catabolize the citrate into oxaloacetic acid and acetic acid. The oxaloacetic acid is then broken down into pyruvate and the acetic acid is converted to CO2. The CO2 reacts with the water and sodium in the media to produce alkaline sodium carbonate.
![Citrate Utilization](http://iws2.collin.edu/dcain/CCCCD%20Micro/Citrate.jpg)
Simmon's Citrate agar slants contain sodium citrate (the only carbon source). Thus, a positive result for citrate utilization is the formation of a BLUE color.
Citrate, a Krebs cycle (i.e., TCA cycle or citric acid cycle) intermediate, is generated by many bacteria; however, utilization of exogenous citrate requires the.
Citrate utilization plasmids have previously been identified in atypical Escherichia coli isolates. A different citrate-utilizing (Cit+) variant of E. coli K-12 arose.
Identification of Bacteria: Citrate Utilization Test. Introduction There are a series of biochemical tests which are useful for the identification.
Citrate Utilization In Bacteria
Citrate agar slants contain sodium citrate, bromthymol blue (pH indicator), sodium, and water. Tube 2: Positive for citrate utilization.